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Título
Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Pathogens
Número de la revista
1
Cita Bibliográfica
Ramírez, H., Echeverría, I., Benito, A. A., Glaria, I., Benavides, J., Pérez, V., de Andrés, D., & Reina, R. (2021). Accurate diagnosis of small ruminant lentivirus infection is needed for selection of resistant sheep through tmem154 e35k genotyping. Pathogens, 10(1), 1-16. https://doi.org/10.3390/PATHOGENS10010083
Editorial
MDPI
Fecha
2021
Resumen
[EN] Small ruminant lentiviruses (SRLV) cause an incurable multiorganic disease widely spread in sheep and goats that disturbs animal welfare and production. In the absence of a vaccine, control measures have been traditionally based on early diagnosis and breeding with virus-inactivated colostrum with segregation of seropositive animals. However, antigenic heterogeneity, poor antibody production due to low viral load, and single strain design of most available ELISA, pose a threat to SRLV diagnosis. Genome-wide association studies have described TMEM154 E35K polymorphism as a good genetic marker for selection of resistant animals in some American and European breeds. In this study, a multitargeted serological and virological screening of more than 500 animals from four different breeds (latxa, raza Navarra, assaf, and churra) attending to SRLV infection status was performed. Then, animals were genotyped to characterize TMEM154 E35K polymorphism. ELISA procedures, individually considered, only identified a proportion of the seropositive animals, and PCR detected a fraction of seronegative animals, globally offering different animal classifications according to SRLV infection status. TMEM154 allele frequency differed substantially among breeds and a positive association between seroprevalence and TMEM154 genotype was found only in one breed. Selection based on TMEM154 may be suitable for specific ovine breeds or SRLV strains, however generalization to the whole SRLV genetic spectrum, ovine breeds, or epidemiological situation may need further validation
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Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad / RTI2018-096172-B-C31/ES/ ADYUVANTES VACUNALES ALTERNATIVOS EN ENFERMEDADES INFECCIOSAS OVINAS PARA HACER FRENTE A LOS EFECTOS ADVERSOS DERIVADOS DEL HIDROXIDO DE ALUMINIO COMO ADYUVANTE//
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