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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Ramírez, Hugo | |
dc.contributor.author | Echeverría, Irache | |
dc.contributor.author | Benito, Alfredo A. | |
dc.contributor.author | Glaria, Idoia | |
dc.contributor.author | Benavides, Julio | |
dc.contributor.author | Pérez Pérez, Valentín | |
dc.contributor.author | de Andrés, Damián | |
dc.contributor.author | Reina, Ramsés | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2021 | |
dc.date.accessioned | 2024-03-12T11:26:39Z | |
dc.date.available | 2024-03-12T11:26:39Z | |
dc.identifier.citation | Ramírez, H., Echeverría, I., Benito, A. A., Glaria, I., Benavides, J., Pérez, V., de Andrés, D., & Reina, R. (2021). Accurate diagnosis of small ruminant lentivirus infection is needed for selection of resistant sheep through tmem154 e35k genotyping. Pathogens, 10(1), 1-16. https://doi.org/10.3390/PATHOGENS10010083 | es_ES |
dc.identifier.other | https://www.mdpi.com/2076-0817/10/1/83 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/18836 | |
dc.description.abstract | [EN] Small ruminant lentiviruses (SRLV) cause an incurable multiorganic disease widely spread in sheep and goats that disturbs animal welfare and production. In the absence of a vaccine, control measures have been traditionally based on early diagnosis and breeding with virus-inactivated colostrum with segregation of seropositive animals. However, antigenic heterogeneity, poor antibody production due to low viral load, and single strain design of most available ELISA, pose a threat to SRLV diagnosis. Genome-wide association studies have described TMEM154 E35K polymorphism as a good genetic marker for selection of resistant animals in some American and European breeds. In this study, a multitargeted serological and virological screening of more than 500 animals from four different breeds (latxa, raza Navarra, assaf, and churra) attending to SRLV infection status was performed. Then, animals were genotyped to characterize TMEM154 E35K polymorphism. ELISA procedures, individually considered, only identified a proportion of the seropositive animals, and PCR detected a fraction of seronegative animals, globally offering different animal classifications according to SRLV infection status. TMEM154 allele frequency differed substantially among breeds and a positive association between seroprevalence and TMEM154 genotype was found only in one breed. Selection based on TMEM154 may be suitable for specific ovine breeds or SRLV strains, however generalization to the whole SRLV genetic spectrum, ovine breeds, or epidemiological situation may need further validation | es_ES |
dc.language | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Sanidad animal | es_ES |
dc.subject.other | Small ruminant lentiviruses | es_ES |
dc.subject.other | TMEM154 | es_ES |
dc.subject.other | ELISA | es_ES |
dc.subject.other | PCR | es_ES |
dc.title | Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.3390/PATHOGENS10010083 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad / RTI2018-096172-B-C31/ES/ ADYUVANTES VACUNALES ALTERNATIVOS EN ENFERMEDADES INFECCIOSAS OVINAS PARA HACER FRENTE A LOS EFECTOS ADVERSOS DERIVADOS DEL HIDROXIDO DE ALUMINIO COMO ADYUVANTE// | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 2076-0817 | |
dc.journal.title | Pathogens | es_ES |
dc.volume.number | 10 | es_ES |
dc.issue.number | 1 | es_ES |
dc.page.initial | 83 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.description.project | This research was funded by Spanish Ministry of Science, Innovation, and Universities, grant number RTI2018-096172-B-C31. The APC was funded by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). I.E. was funded by Universidad Pública de Navarra. Hugo Ramírez was financially supported by the “Programa de apoyos para la superación del personal académico (PASPA)” scholarship from DGAPA-UNAM | es_ES |
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