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Título
A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
The Cell Surface
Datos de la obra
van Leeuwe, T. M., Wattjes, J., Niehues, A., Forn-Cuní, G., Geoffrion, N., Mélida, H., Arentshorst, M., Molina, A., Tsang, A., Meijer, A. H., Moerschbacher, B. M., Punt, P. J., & Ram, A. F. J. (2020). A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan. Cell Surface, 6. https://doi.org/10.1016/J.TCSW.2020.100039
Editor
Elsevier
Fecha
2020
ISSN
2468-2330
Résumé
[EN] Chitin is an important fungal cell wall component that is cross-linked to β-glucan for structural integrity.
Acquisition of chitin to glucan cross-links has previously been shown to be performed by transglycosylation
enzymes in Saccharomyces cerevisiae, called Congo Red hypersensitive (Crh) enzymes. Here, we characterized the
impact of deleting all seven members of the crh gene family (crhA-G) in Aspergillus niger on cell wall integrity, cell
wall composition and genome-wide gene expression. In this study, we show that the seven-fold crh knockout
strain shows slightly compact growth on plates, but no increased sensitivity to cell wall perturbing compounds.
Additionally, we found that the cell wall composition of this knockout strain was virtually identical to that of the
wild type. In congruence with these data, genome-wide expression analysis revealed very limited changes in
gene expression and no signs of activation of the cell wall integrity response pathway. However, deleting the
entire crh gene family in cell wall mutants that are deficient in either galactofuranose or α-glucan, mainly α-1,3-
glucan, resulted in a synthetic growth defect and an increased sensitivity towards Congo Red compared to the
parental strains, respectively. Altogether, these results indicate that loss of the crh gene family in A. niger does
not trigger the cell wall integrity response, but does play an important role in ensuring cell wall integrity in
mutant strains with reduced galactofuranose or α-glucan.
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MINECO/ Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/ PCIN-2015-142/ES/ QUITOSANOS FUNGICOS DE MICELIOS FERMENTADOS COMO BIOESTIMULANTES VEGETALES
URI
DOI
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