Compartir
Título
Impact of sperm DNA damage and oocyte-repairing capacity on trout development
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Reproduction
Número de la revista
1
Datos de la obra
Fernández-Díez, C., González-Rojo, S., Lombó, M., & Herráez, M. P. (2016). Impact of sperm DNA damage and oocyte-repairing capacity on trout development. Reproduction, 152(1), 57-67. https://doi.org/10.1530/REP-16-0077
Editor
Society for Reproduction and Fertility
Fecha
2016
ISSN
1470-1626
Abstract
[EN] Zygotic repair of paternal DNA is essential during embryo development. In spite of the interest devoted to sperm DNA damage, its combined effect with defect-repairing oocytes has not been analyzed. Modification of the breeding season is a common practice in aquaculture. This practice reduces developmental success and could affect the both factors: sperm DNA integrity and oocyte repair capacity. To evaluate the maternal role, we analyzed the progeny outcome after fertilizing in-season trout oocytes with untreated and with UV-irradiated sperm. We also analyzed the offspring obtained out of season with untreated sperm. The analysis of the number of lesions in 4 sperm nuclear genes revealed an increase of 1.22–11.18 lesions/10 kb in out-of-season sperm, similar to that obtained after sperm UV irradiation (400 µW/cm25 min). Gene expression showed in out-of-season oocytes the overexpression of repair genes (ogg1, ung, lig3, rad1) and downregulation of tp53, indicating an enhanced repairing activity and reduced capacity to arrest development upon damage. The analysis of the progeny in out-of-season embryos revealed a similar profile tolerant to DNA damage, leading to a much lower apoptotic activity at organogenesis, lower hatching rates and increased rate of malformations. The effects were milder in descendants from in-season-irradiated sperm, showing an enhanced repairing activity at epibolia. Results point out the importance of the repairing machinery provided by the oocyte and show how susceptible it is to environmental changes. Transcripts related to DNA damage signalization and repair could be used as markers of oocyte quality
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MINECO/ Plan Nacional de I+D+i/ AGL2011-27787/Análisis de la cromatina espermática de peces y sus aportaciones al control del desarrollo y la calidad de la descendencia
URI
DOI
Versión del editor
Collections
- Untitled [5567]
Files in this item
Tamaño:
1.250
xmlui.dri2xhtml.METS-1.0.size-megabytes
Formato:
Adobe PDF