Compartir
Título
Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
PLOS ONE
Número de la revista
7
Cita Bibliográfica
Balseiro, A., Altuzarra, R., Vidal, E., Moll, X., Espada, Y., Sevilla, I. A., Domingo, M., Garrido, J. M., Juste, R. A., Prieto, M., & Pérez de Val, B. (2017). Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep. PLoS ONE, 12(7), Article e0180546. https://doi.org/10.1371/JOURNAL.PONE.0180546
Editorial
Public Library of Science
Fecha
2017
Resumen
[EN]Background/Aims: Animal tuberculosis (TB) is a complex animal health problem that causes disruption to trade and significant economic losses. TB involves a multi-host system where sheep, traditionally considered a rare host of this infection, have been recently included. The aims of this study were to develop an experimental TB infection model in sheep with a Mycobacterium caprae field strain isolated from a tuberculous diseased ewe, and to use this to evaluate the safety and efficacy of two vaccines against TB in sheep, the live-attenuated M. bovis BCG vaccine (Danish strain) and a heat-inactivated M. bovis (HIMB) vaccine. Methods: Eighteen 2 month-old lambs were experimentally challenged with M. caprae by the endotracheal route (1.5 × 103 CFU). They were separated per treatment group into parenterally vaccinated with a live BCG Danish strain vaccine (n = 6), orally vaccinated with a suspension of HIMB (n = 6) and unvaccinated controls (n = 6). Clinical, immunological, pathological and bacteriological parameters of infection were measured. Results: All lambs were successfully infected and developed gross TB lesions in the respiratory system. The BCG vaccine conferred considerable protection against experimental TB in lambs, as measured by a reduction of the gross lesion volumes and bacterial load. However, HIMB vaccinated animals did not show protection. Conclusions: This study proposes a reliable new experimental model for a better understanding of tuberculosis in sheep. BCG vaccination offers an effective prospect for controlling the disease. Moreover alternative doses and/or routes of administration should be considered to evaluate the efficacy of the HIMB vaccine candidate.
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTA2014-00002-C02-01/ES/Estrategias de control de la tuberculosis bovina en reservorios silvestres (tejón y jabalí) y domésticos (ovino)
URI
DOI
Versión del editor
Aparece en las colecciones
- Artículos [5498]
Ficheros en el ítem
Tamaño:
3.929
xmlui.dri2xhtml.METS-1.0.size-megabytes
Formato:
Adobe PDF