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    Citas

    Título
    Detection of rainbow trout antibodies against viral haemorrhagic septicaemia virus (VHSV) by neutralisation test is highly dependent on the virus isolate used
    Autor
    Fregeneda Grandes, Juan MiguelAutoridad BuleriaORCID
    Olesen, Niels Jørgen
    Facultad/Centro
    Facultad de Veterinaria
    Área de conocimiento
    Sanidad Animal
    Datos de la obra
    Diseases of aquatic organisms, 2007, vol. 74, n. 2
    Editor
    Inter-Research
    Fecha
    2007-02-28
    Abstract
    Three serological tests, enzyme linked immunosorbent assay (ELISA), 50% plaque neutralisation test (50%PNT) and Western blotting (WB), were used to detect antibodies against viral haemorrhagic septicaemia virus (VHSV) in 50 rainbow trout broodstock from a rainbow trout farm endemically infected with VHS but with no clinical signs of infection. When the sera were examined by 50%PNT using the VHSV reference isolate DK-F1 or the heat attenuated DK-F25 mutant strain, no neutralizing antibodies were found. In contrast, when one of the virus isolates from the farm (homologous virus) was used in the 50%PNT, 90% of the fish were found to be positive. By examining a panel of different VHSV isolates in 50%PNT, it was demonstrated that the virus isolate used as test antigen could significantly affect the sensitivity and titre determination in 50%PNT for detection of rainbow trout antibodies against VHSV. When the sera were examined for the presence of VHSV antibodies by ELISA or WB, 61% were found to be positive. When conducting WB analysis, the viral glycoprotein was the protein most frequently recognized, followed by the viral nucleoprotein.
    Materia
    Inmunología
    Sanidad animal
    Veterinaria
    Zoología
    Palabras clave
    Truchas
    Serología
    Inmunoglobulinas
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/9024
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